Triton X 100 in PBS, saturated with 3 bovine serum albumin, and incubated with primary antibodies at room temperature for 1 h, followed by rhodamine labeled secondary antibodies Sigma Aldrich Italia, Milano, Italy and then by Hoechst dye
propecia viagra combined However, despite a decrease in pyruvate release in the PIK3CA mutant cells, both glucose consumption and glucose derived lactate release remained unaffected, suggesting that any alteration of the fate of pyruvate was limited to either the fraction entering mitochondria or pyruvate converted to alanine